Journal: Frontiers in Neuroscience

Article Title: Modeling Protein Aggregation and the Heat Shock Response in ALS iPSC-Derived Motor Neurons

doi: 10.3389/fnins.2018.00086

Figure Lengend Snippet: Antibodies.

Article Snippet: Mouse α-Islet1 , DSHB , 40.2D6.

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Modeling Protein Aggregation and the Heat Shock Response in ALS iPSC-Derived Motor Neurons

doi: 10.3389/fnins.2018.00086

Figure Lengend Snippet: iPSC-derived motor neurons from multiple ALS genotypes do not exhibit reduced viability in culture. (A) Representative images at 3 weeks of differentiation of control and ALS motor neurons immunostained for Tuj1 (green), ChAT (red), and Islet1 (white). Nuclei are labeled with Hoechst nuclear dye (blue). Scale bar = 50 μm. (B) Quantification shows no difference in the number of motor neurons at 3 or 4 weeks of differentiation. n = 3–4. Not significant by one-way ANOVA with Tukey post-hoc test.

Article Snippet: Mouse α-Islet1 , DSHB , 40.2D6.

Techniques: Derivative Assay, Control, Labeling

Journal: Diabetes

Article Title: MafA and MafB Regulate Genes Critical to β-Cells in a Unique Temporal Manner

doi: 10.2337/db10-0190

Figure Lengend Snippet: The mRNA expression profile of E18.5 wild-type and mutant pancreata

Article Snippet: The primary antibodies used were guinea pig α-insulin (1:2,000; Linco Research), mouse α-insulin (1:2,000; Invitrogen), guinea pig α-glucagon (1:2,000; Linco Research), sheep α-somatostatin (1:2,000; American Research), guinea pig α-pancreatic polypeptide (1:2,000; Linco Research), rabbit α-MafB (1:10,000; Bethyl Laboratories), rabbit α-MafA (1:1,000, Bethyl Laboratories), rabbit α-Slc30a8 (1:1,000, Mellitech), mouse α-Rbp4 (1:1,000, Abnova), rabbit α-G6PC2 (1:500, a gift from Dr. John Hutton at the University of Colorado), rabbit α-Pdx1 (1:5,000, a gift from Dr. Chris Wright at Vanderbilt University), mouse α-Isl1 (1:300, Developmental Studies Hybridoma Bank), rabbit α-Nkx6.1 (1:1,000, Beta Cell Biology Consortium), and rabbit α-Pax6 (1:200, Covance Research Products).

Techniques: Expressing, Mutagenesis